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HouYuan Autoclave for Mushroom Cultivation
Sep 24, 2021
How to Cultivate Mushrooms with HouYuan Autoclave Equipment?


Nationwide, we’re eating more and more mushrooms every single year. Not just the bland, soggy ones from the corner store, either. We’re talking specialty types like oyster, shiitake, even mistake and lion’s mane.


And it’s surprisingly easy to get started—provided you’ve got the HouYuan autoclave machine for mushroom cultivation. Here’s everything you need to know to prepare your first batch:
1. As usual, decide which type of mushroom you’ll cultivate.
2. Prepare the grain (typically rye berry, millet, wheat, or corn) by soaking it for 24 hours, then cleaning and drying it.
3. Place the grain in jars or grow bags with an air filter patch and injection port.
4. Load the jars or bags in autoclave equipment for mushroom cultivation.
5. Set the autoclave’s timer as your batch size dictates. A typical range is 1-2 hours at 121°C and 15 psi.
6. Inoculate the sterilized grain via grain spawn transfer, agar tissue culture transfer, or liquid inoculation with a syringe.
7. Wait a few days as the grain gets fully colonized with mycelium and develops mushroom spawn.
8. Mix the grain spawn with the pasteurized substrate, and sit back as it grows into big, flavorful mushrooms!


Should you have any requirements, please feel free to contact us. Portable autoclave, vertical autoclave, table top autoclave and horizontal autoclave are available.

autoclave machine for mushroom cultivation

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